Phenotype reports: Sharing the knowledge

Every single story has a beginning, and “Phenotype Reports” is no exception. Its conception dates back to couple of years ago and, as many other fruitful collaborations, it precisely started during congress “after dinner.” I (Genny Del Zotto) was speaking with Prof. Preijers Tárnok remembering that at the beginning my working experience in Hospital Core Facility different users came me asking for new panels distinguish cell populations species that, some cases, barely knew. In first period, had awfully hard times trying find best antibody clones fluorescence each molecule interest, even sifting through literature, frequently found controversial opinions. For sure, made lot mistakes but, we all know well, “we learn from our mistakes” now, laboratory, have developed ready use can be applied experimental diagnostic settings. Similar experiences, course, happened us opinion, this kind information, together process helped researchers design these should spread around help youngest scientific community members avoid already by others. With mind, desire helping approaching population, think about way create topic could give necessary information on given its most representative markers simplest schematic be, same time, linked well-known Optimized Multicolor Immunophenotyping Panelb(OMIP) protocols1 celebrated last year their 10th anniversary.2 OMIP papers are short focused. Therefore, there space explaining detail why particular combination antigens needs detected antibodies identify specific population (and characterize function). This not unique OMIPs but articles high-dimensional labeling. Few months after conversation, three decided publish collection state-of-the-art phenotype reports write editorial present ideas Cytometry immunology community,3 define guidelines authors, introduce prototype Phenotype Report.4 Report paper concise form identifying peripheral blood or organs one species. particular, show, critical comparison weighting recent literature own result, which antigen combinations essential characterization type. Gating strategies, detailed tables molecules, an exhaustive description procedure (MIFlowCyt) sharing example files public flow repository.5 key points report What always important when simple clear population's basic functions precise idea what every phenotyping molecule's role is. Given above-mentioned premises, ideal candidate authors writing who published recently hot-topic research using complex immunophenotyping panels. We proud here Reports. Mair Liechti (this issue includes pp. 231-242) explain rational they used OMIP-0446 dendritic (DC) compartments. They provide comprehensive overview classic consensus strategies human DCs Monocytes relate approaches studies redefined heterogeneity phenotypes blood.7-10 Particular attention been unravel phenotypic differences between cDC2 inflammatory various monocyte subsets single-cell characterizations. An additional aspect DC biology focus second report. dedicated changes tumoral environment. Carenza et al. 218-230) describe phases cancer immunoediting, namely: elimination, equilibrium, escape.11 Since play pivotal elimination equilibrium tumor progression,12 knowledge activation state greatly understanding diagnosing possible disease progression also aid choice eventual therapies. The schemes occur contexts useful technical advises TIM-3 characterization. two following reviews, Grant murine 251-256) endothelial cells 257-264), respectively. Both unequivocal circulating population.13 moving Mousset al.4 “Comprehensive Phenotyping T Cells Using Flow Cytometry” another species, (GDZ) analysis pp.). line colleagues, intended only main T-cell subpopulations since more regulatory helper scheduled year. conclusion, Reports represents philosophy behind topic, whose purpose trace guidelines, tip, tricks address consistent reproducible population. Papers such those presented perfect starting point fields time. Their importance depends segregation explained bibliography carefully selected represent art. Genny Zotto: Conceptualization; writing-original draft; writing-review & editing. Frank Preijers: Attila Tarnok: